Background &objectives:The present study was to carry out 90 days repeated oral toxicity of Urai mathirai in wistar albino rats. Methods:The chronic toxicity study was carried out as per (OECD) test guideline 408 and Central Council for Research in Ayurvedic Sciences (CCRAS) respectively. In this study, theUrai mathirai drug solution was administered at dose of (10, 50 and 100mg/kg b.wt) for every 24 hours orally upto 90 days for all the three groups. At the end of each study, hematological and biochemical analysis were evaluated. Histopathological examination of vital organs of the rats were taken for gross findings, compared to controls. Distilled water aided as a control in all the tests. Results: There was no significant difference (p > 0.05) observed in the relative organs weight, body weight, hematological, biochemical parameters, and gross abnormalities when compared to the control. No mortality was recorded with respect drug effect. Interpretation&conclusions: The results may lead that the oral administration of the Urai mathiraiat doses of 10, 50 and100 mg/kg.b.wtdoes not showedany toxicity effect in wistar rats during the experimental period. Keywords: Urai mathirai; chronic toxicity; herbal formulation; histopathology.