A collagen thin film-based bioimpedance sensor for cell proliferation
rate assessment
Abstract
In this paper, a bioimpedance sensor used to measure the impedance
behavior of the biological cells cultured on a scaffold of collagen thin
films. The collagen thin films with different concentrations were
created on a 1-hexadecanethiol modified surface of the interdigitated
electrodes(IDEs). The mesenchymal stem cells(MSCs) and human umbilical
vein endothelial cells(HUVECs) were cultured on the collagen thin films.
The interaction and the proliferation rate of these cells were
investigated by the microscopic, and bioimpedance approaches. Results
showed that the MSCs, and HUVECs excellently attached to the collagen
thin films. Impedance measurements of the cells cultured on the collagen
thin film were performed by a Hioki IM3570 impedance analyzer at the
frequency band of 10 kHz to 1MHz at 10 mV. Bioimpedance measurements
showed that the proliferation rate of both MSCs, and HUVECs increased by
decreasing the collagen thin films concentration. After 48 h, the
differential impedances (ΔΖ ) of the MSCs cultured on the collagen thin
layers with concentrations of 250μg/mL,500μg/mL , and 1000μg/mL measured
3.09Ω≤Δ≤48.03Ω, 2.62Ω≤Δ≤45.79Ω, and 1.9Ω≤Δ≤25.22Ω, respectively. Also,
ΔΖ of the HUVECs obtained 27.3Ω≤Δ≤39.2Ω, 8.25Ω≤Δ≤18.43Ω, and
0.96Ω≤Δ≤9.6Ω for the corresponding frequency band and collagen thin
films concentrations.