Nanotechnology deals with the study and manipulation of materials on an atomic or a molecular scale almost less than 100 nm. Nanotechnology encompasses the synthesis and applications of biological, chemical and physical systems which ranges from individual atom to molecular or submicron level. It is the more interesting field for the scientists due to various applications in different fields of science e.g., biomedical engineering, material science, medicine and electronics at nanoscale level. The particles ranges from 1 to 100 nm by a surrounded interfacial layer are basically called nanoparticles. Ag nanoparticles are the most fascinating and vital nanoparticles amongst the other metallic nanoparticles like gold, tin, palladium etc. In this review literature, the synthesis and properties of Ag nanoparticles have been discussed. Herein, main area of study interest is to find biomolecules by using conventional as well as colorimetric detection methods with silver nanoparticles. Synthesis and characterization of Ag nanoparticles is an immense attention in nanotechnology due to its wide range applications in industries. Ag nanoparticles are synthesized by different methods (physical, chemical, biological and photochemical methods), amongst these methods green synthesis method is most favorable method, because it is cost effective and eco friendly method. Ag nanoparticles have various significant properties such as antibacterial, antifungal, antiviral, anti-cancer, and anti-angiogenic etc. Nanoparticles possess many applications in different fields of life e.g., medicine, biomedical devices, multicolor optical coding, manipulation of biomolecule (proteins, DNA, cysteine), environmental remediation and cosmetics. The aim of prospective applications is to investigate the colorimetric and optical detection of biomolecule by Ag nanoparticles, e.g., dopamine, proteins, DNA etc. because of its highly sensitivity and selectivity. The colorimetric detection of various biomolecules by Ag nanoparticles has inherent chirality. Ag nanoparticles provides high surface to volume ratio and function for detection of proteins, nucleic acid, ions and molecules. Ag nanoparticles as colorimetric probes and chiral selector are helpful in determining citalopramenantiomers. However, we can easily read out the assay described in this work with naked eye and by use of ultra violet spectrometer. [Shahzad Sharif Mughal, Faheem Abbas, Muhammad Usman Tahir, Ali raza ayub, Hafiza Maria javed, Muhammad mamtaz, Hafiza iram. Role of Silver Nanoparticles in Colorimetric Detection of Biomolecules. Biomedicine and Nursing 2019;5(4): 31-47].