Figure 4: Validation of miRNA biomarkers in patients with atopic dermatitis before and after systemic therapy compared to healthy individuals. Validation of selected miRNA candidates using RT-qPCR. The panel shows miRNA levels in AD patients (paired samples before and after therapy), psoriasis patients, and healthy individuals. Comparisons between unpaired groups were done using the Mann-Whitney U test and, in paired groups, using the Wilcoxon test.