Observations
RNA localization images with immunofluorescence are accurately and
clearly described. Alternative explanations for probe localization such
as RNA-DNA hybrids are properly acknowledged with RNase control
experiments. However, in the case of the cenRNA-CENP-B co-IF images,
there is no mention or explanation of apparent nonspecific staining that
is occurring in the đź-satellite (red) channel, which could make these
figures harder to interpret on a first read-through. For readers who are
non-experts in IF techniques, descriptions of IF images in Figures 2A
and 3B will be easy to follow, however the authors may consider
elaborating on what they consider to be a âjuxtaposedâ localization of
cenRNAs and CENP-B in reference to their model, and whether this was a
general observation.