Culture of hiPSC-derived pancreatic cells
hiPSC-derived pancreatic cells (day 12 of differentiation) were seeded on scaffolds with two different modifications: amine-modified and gelatin-coated, NaOH-modified. Cells were seeded either as single cells or as aggregates. For seeding single cells, the aforementioned method was followed. To seed the aggregates, cell islets with an average of 4000 cells were detached manually from wells of a 6-well plate by a 10 µl pipette tip under microscopic observation and were transferred to a 15 ml centrifuge tube followed by gravitational settling. After 10 minutes at 37° C, the supernatant was removed, and aggregates were resuspended in 360 µl of stage-four medium (DMEM/F12, B27, p/s). 10 µl of cell suspension, containing 5 aggregates was added to each prepared scaffold. The same differentiation protocol was followed for cells grown on scaffolds as in dishes.