Culture of primary pancreatic cells
Primary pancreatic cells were removed from culture flasks by incubation with trypsin-EDTA (0.25%) for 5 minutes. Cells were centrifuged at 100 x g for 5 minutes; the supernatant was removed, and 10 µl of cell suspension containing 20,000 cells was added to each scaffold. After 4 hours, 100 µl culture media was added to each well. To maintain pancreatic cells on scaffolds, medium was changed every other day for 10 days. Six replicate cultures of all six scaffold modifications were performed for each experiment.