High glucose but not STZ increased heparanase expression in cultured islets
In vitro, chronic (24 h) incubation in glucose dose-dependently stimulated heparanase expression in cultured islets (Fig. 6E), whereas STZ treatment did not altered heparanase expression (Fig. 6F). We further screened the mRNA expression of transcriptional factors that were associated with the mRNA transcription of Heparanase . After a 24 h incubation with 25 mmol/L glucose, the expression of Ppargwas reduced, while p53 , Egr1 , and Ets1 were increased when compared with islets treated with 11.1 mmol/L glucose (Fig. 6H). By contrast, the expression of Smad4 , Lef1 andTgli1 was not altered by incubation with 25 mmol/L glucose for 24 h (Fig. 6G).