Luciferase reporter gene assay
Truncated heparanase promoters (-2000–0, -1000–0, -300–0) were cloned forward of the luciferase reporter gene in a pGL3 basic vector, which was co-transfected with PPARγ vectors into 293T cells using Lipofectamine 2000 (Invitrogen). Luciferase activity was detected with the Luciferase Reporter Assay System (Promega, Fitchburg, WI, USA), as previously described (Li et al. , 2019).