Cell culture and treatment
Cell culture reagents were all purchased from Gibco (Oakland, CA, USA). MIN6 cells [BCRJ Cat#0293, RRID: CVCL_0431] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 25.5 mM glucose and 10% fetal bovine serum (FBS) and antibiotics in a 37°C incubator with 5% CO2. STZ (10 mM) was added to the culture medium of MIN6 cells in the presence of different doses of OGT2115. For luciferase reporter gene assays, 293T cells were cultured in high glucose DMEM with 10% FBS at 37°C in 5% CO2. For cell viability assays, MIN6 cells in 96-well plates were divided into different treatment groups (five replicated wells). After treatment, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) was used to measure cell viability, as previously described (Sunet al. , 2016).