Experimental system
Autoxidation experimental design was based on previous studies (Resende
et al. 2019ab; Weisman et al., 2018; Berman et al., 2016; Meiri et
al., 2015).
Briefly, six samples of 250ml of linseed oil in 500ml beakers were
heated in a hot plate at different temperatures (25, 40, 60, 80, 100 and
120 °C). Air was pumped into the beaker with maximum magnetic stirring
for up to 168 hours. Before the induced autoxidation process (time 0)
and after time periods of 3, 6, 9, 24, 48, 72, 96, 120 and 168 hours of
heating, a 20 mL sample was removed into a vial which was placed in cold
water for 10 min and then stored sealed at −20 °C to avoid further
oxidation prior to analysis.