IL-35 expression affected the gene expression profiles
We stratified both the tumor (249 cases) and non-tumor (239 cases)
samples into IL-35 low and high groups according to its gene expression
level. Then, class comparison analyses of transcriptome data between
IL-35-low and -high groups were performed to investigate the changes in
mRNA expression profiles. A total of 1625 differentially expressed genes
(DEGs) were found (univariate P < 0.001, false discovery rate
< 0.05; Fig.3B) in tumor samples, whereas 4238 DEGs were
detected in the non-tumor group (Fig.3A). Venny plot analysis showed
that there were a total of 1270 genes changed in both tumor and
non-tumor tissues (Fig.4A). To determine the biological functions for
these genes, we performed IPA analysis for the overlapped 1270 gene list
and found that the top 20 [log(p-value) > 5] signal
pathway were immune function related, especially the T cell pathway
(Fig.4B).