Disclaimers
Assay/system dependence: If the system or assays change, the same ligand and receptor may display a very different pathway-preference. However, the same may actually be true even when using a reference ligand, as that does not equate any imbalance in pathways, but merely move the baseline. Imbalance in pathways is instead predominantly determined by the choice of systems and assays, which should be as similar as possible.
Fold potency: Fold potency comparisons without the inclusion of efficacy are merely to be seen as a prediction of potential pathway-preference or bias, as in case the efficacies were known, the real relationships may be very different.