Figure Legends
Figure 1 : Effect of BSO treatment on cell growth, productivity
and GSH content. Gray and blue indicates the feed medium used –
control feed medium or low cysteine feed medium, respectively. Circles
indicates non-treated bioreactors and triangles indicates BSO-treated
bioreactors. The red dotted lines represent the timing of BSO addition
to a medium concentration of 0.5mM. (A) viable cell concentration (VCC)
profile. (B) Product titer in the supernatant over time. (C)
Intracellular GSH concentration overtime. (D) mAb Specific productivity
over time.
Figure 2 : Effect of BSO treatment on glucose, lactate and
alanine uptake/secretion rates. Gray and blue indicates the feed medium
used – control feed medium or low cysteine feed medium, respectively.
Circles indicates non-treated bioreactors and triangles indicates
BSO-treated bioreactors. The red dotted lines represent the timing of
BSO addition to a medium concentration of 0.5mM. (A) Glucose rate (B)
Lactate rate (C) Alanine rate.
Figure 3 : Heatmap of differentially expressed proteins under
BSO treatment in CHO cells. Main cellular function of proteins cluster
has been added in the figure.
Figure 4 : Glutathione metabolism related proteins
expression in BSO-treated cells. The logFC and the adj. p. valueare represented by the color from green to red. Red indicates a
logFC>0.5 and a adj. p. value <0.05 ; ligh
red indicates logFC<0.5 and a adj. p. value<0.05; green indicates a logFC<-0.5 and a adj.
p. value <0.05; light green indicates logFC>-0.5
and a adj. p. value <0.05 and grey adj. p. value>0.05. Statistical data have been generated using limma
empirical Bayes moderated t-test. The BSO-treated condition (n=10) have
been compared to control conditions (n=12).