Figure Legends
Figure 1 : Effect of BSO treatment on cell growth, productivity and GSH content. Gray and blue indicates the feed medium used – control feed medium or low cysteine feed medium, respectively. Circles indicates non-treated bioreactors and triangles indicates BSO-treated bioreactors. The red dotted lines represent the timing of BSO addition to a medium concentration of 0.5mM. (A) viable cell concentration (VCC) profile. (B) Product titer in the supernatant over time. (C) Intracellular GSH concentration overtime. (D) mAb Specific productivity over time.
Figure 2 : Effect of BSO treatment on glucose, lactate and alanine uptake/secretion rates. Gray and blue indicates the feed medium used – control feed medium or low cysteine feed medium, respectively. Circles indicates non-treated bioreactors and triangles indicates BSO-treated bioreactors. The red dotted lines represent the timing of BSO addition to a medium concentration of 0.5mM. (A) Glucose rate (B) Lactate rate (C) Alanine rate.
Figure 3 : Heatmap of differentially expressed proteins under BSO treatment in CHO cells. Main cellular function of proteins cluster has been added in the figure.
Figure 4 : Glutathione metabolism related proteins expression in BSO-treated cells. The logFC and the adj. p. valueare represented by the color from green to red. Red indicates a logFC>0.5 and a adj. p. value <0.05 ; ligh red indicates logFC<0.5 and a adj. p. value<0.05; green indicates a logFC<-0.5 and a adj. p. value <0.05; light green indicates logFC>-0.5 and a adj. p. value <0.05 and grey adj. p. value>0.05. Statistical data have been generated using limma empirical Bayes moderated t-test. The BSO-treated condition (n=10) have been compared to control conditions (n=12).