Physical observations
The body weights of the mice in each group (NC, XC, and PP242-treated)
were measured once in every two days and compared (Fig. 1). Compared to
the NC group, the body weights of the XC group mice decreased, but the
changes were not significant. In contrast, the body weights of the
PP242-treated mice decreased significantly and quickly in the first
couple of days after treatment compared to the NC group, and later, the
body weights gradually recovered. However, no significant change in body
weight was observed between the XC and PP242-treated groups, indicating
that the change in tumor size and drug treatment did not seriously
affect body weight within 21 days.
Tumor-bearing mice were treated with vehicle and/or PP242 over the
course of 21 days, and the size of the tumor in the right flank region
was measured at two day intervals using digital caliper in the LS174T
xenograft groups (XC and PP242-treated) in order to evaluate the drug
therapeutic effects in vivo . After two weeks of PP242 treatment,
a very significant difference in the tumor volumes was observed
(p<0.001) before the mice were sacrificed (Fig. 2A and 2B).
The tumors were separated after sacrifice and weighed, which further
displayed the significant decrease in tumor weight for the PP242
treatment compared to the XC group mice (Fig. 2C).