Fig. 8. Overview of affected pathways of identified 59 significantly altered metabolites after the treatment with second generation mTOR inhibitor (PP242) at an oral dose of 60 mg.kg-1.day-1 for three weeks. The major perturbed metabolic pathways associated with PP242 effects were energy metabolism, fatty acid metabolism and glycerophospholipid metabolism. Grey color represents increased metabolites after the mTOR inhibition by PP242; dark grey color represents decreased metabolites after the mTOR inhibition by PP242 [grey and dark grey box indicates the identified metabolites that are significantly altered].