2.2 Detection of SARS-CoV-2
200 µl sample supernatant was used for extraction of nucleic acids in
GeneRotex 96 nucleic acid extraction instruments (TianLong Technology,
Xi’an, China) in the Biosafety level 2 laboratory. Then RNA detection
was done using qTower3G thermocycler (Analytikjena,
Germany) by real-time RT-PCR methods. The primers and probes targeting
the N and Orf1ab genes of SARS-CoV-2 were used (Zhu et al., 2020). The
amplification was performed with the One Step PrimeScript™ RT-PCR kit
(TaKaRa, Japan). The amplification condition was as follows: 42°C for 5
min, 95°C for 10 s, followed by 45 cycles of 95°C for 5 s and 58°C for
30 s.