Figure Legends
Fig. 1. Schematic indicating the coordinate system and modeling
dimensions in the flow cell. The schematic is not to scale.
Fig. 2. Schematic of experimental set up, including biofilm
flow cell and OCT unit. The flow was driven by gravity.
Fig. 3. Averaged viscoelastic moduli for synthetic biofilm and
homogenized P. aeruginosa biofilm. The error bars show standard
deviations from 3 replicates.
Fig. 4. Rheometer data and fitted Maxwell model for (a)
synthetic biofilm, and (b) homogenized biofilm. The synthetic biofilm
was tested under a constant strain of 0.01% and the homogenized biofilm
was under 0.1% of strain. The black square markers indicate the
averaged measured shear stress over time. The error bars indicate one
standard deviation, based on the 10 replicates. The green line shows the
fitted Maxwell model.
Fig. 5. Biofilm geometries in the experiment and model. (a)
Cross-sectional OCT image of synthetic biofilm; (b) modeling geometry
based on the OCT image.
Fig. 6. The comparison of synthetic biofilm boundaries. Flow
was from left to right. Green line: biofilm contour at t=0 (in
experiment and computational model); black line: biofilm contour at t=20
s (in experiment); black dashed line: biofilm contour at t=20 s (in
computational model). Unrelated data were filtered out.
Fig. 7. (a) Locations of measurement (lines 1-3) for DIC
analysis. (b)-(d) Experimental and modeling displacement of synthetic
biofilm along lines 1-3.
Fig. 8. Simulated velocity u and stress distribution
for synthetic biofilm at t=2 s. (a) Velocity field of solvent domain
(colored surface and black arrows); (b) velocity field of synthetic
biofilm domain (colored arrows); (c) the magnitude of viscous stress
tensor \(\left|\left|\mathbf{\tau}\right|\right|\ \)on synthetic
biofilm domain; (d) the magnitude of elastic components of the
deviatoric stress tensor \(\mathbf{||\sigma}_{e}\mathbf{||}\) on
synthetic biofilm domain.
Fig. 9. (a) Locations of measurement (lines 1-3) for DIC
analysis. (b)-(d) Experimental and modeling displacement ofP.aeruginosa biofilm along lines 1-3.