Figure 5. Localization of GeSUT4
expression.
(a) Subcellular localization of GeSUT4-GFP fusion proteins in
Arabidopsis protoplasts. Fluorescence images derived from GeSUT4-GFP
fusions, a membrane marker,
AtPIP2A-RFP fusions, and both were shown. The latter “c” and arrowhead
indicate chloroplasts and co-localized signals. (b) Co-localization ofGe SUT4-GFP proteins with a tonoplast marker, AtγTIP-RFP fusions.
(c) Cell-specific expression of GeSUT4 analyzed by in situ
hybridization. Cross-sections of G. elata tubers in Fig 2a were
hybridized with GeSUT4- specific sense and anti-sense probes.
Asterisks (*) indicates locations of strong signals observed. Scales are
10 µm in (a) and 100 µm in (c).