Figure 5. Localization of GeSUT4 expression.

(a) Subcellular localization of GeSUT4-GFP fusion proteins in Arabidopsis protoplasts. Fluorescence images derived from GeSUT4-GFP fusions, a membrane marker,
AtPIP2A-RFP fusions, and both were shown. The latter “c” and arrowhead indicate chloroplasts and co-localized signals. (b) Co-localization ofGe SUT4-GFP proteins with a tonoplast marker, AtγTIP-RFP fusions. (c) Cell-specific expression of GeSUT4 analyzed by in situ hybridization. Cross-sections of G. elata tubers in Fig 2a were hybridized with GeSUT4- specific sense and anti-sense probes. Asterisks (*) indicates locations of strong signals observed. Scales are 10 µm in (a) and 100 µm in (c).