Sampling and laboratory protocols
We sampled tail tips of 29 specimens of the Floreana lava lizard and 33
of the Floreana leaf-toed gecko from Floreana (n = 10 and 10,
respectively), Champion (n = 9 and 10), and Gardner (n = 10 and 13)
islands in August 2018. In addition to the newly generated DNA
sequences, we obtained from GenBank sequences of taxa belonging to the
same radiations as M. grayii and P. baurii , with M.
occipitalis and P. reissii , respectively, as outgroups
(Benavides, Baum, Snell, Snell, & Sites, 2009; Torres-Carvajal et al.,
2014). Lava lizards were captured by noosing or by hand, while geckos
were captured by hand. Samples were stored in Longmire buffer. Floreana
island (173 km2) lies in the south of the Galápagos
archipelago; the islet of Champion (0.094 km2) lies
700 m NE of Floreana, whereas Gardner (0.81 km2) lies
8 km E of Floreana (Fig. 1). Maximum sea depth between Floreana and
these islets is 50 m and 200 m, respectively.
We generated novel DNA sequences of NADH dehydrogenase subunit 2
(ND2) for lava lizards and NADH dehydrogenase subunit 4 (ND4) for
leaf-toed geckos (Genbank accession numbers to be provided) following
published protocols (Kizirian, Trager, Donnelly, & Wright, 2004;
Torres-Carvajal, Barnes, Pozo‐Andrade, Tapia, & Nicholls, 2014).