List of Figure and Table Captions
Figure 1. Schematic diagram of wild type Cel9B and truncation mutants constructed for this study. Sp; signal peptide, E; Eset domain, CBM; carbohydrate binding motif, CD; catalytic domain. Numbers on the top horizontal bar represent amino acid residues.
Figure 2. Study depicting activity profiles of WT and truncation mutants of TfCel9B using CMC as a substrate. For each time course, increasing concentrations of TfCel9B were incubated on 1 mg/ml CMC in 0.6 mL 50 mM sodium acetate used as buffer and µmoles of product were released. Solid lines were derived using logarithm expression. Upper portion, activity profiles of mutants Fn3 and ΔFn3; middle portion, mutants E9B, ΔE9B and cd9B. Bottom portion; WT, mutants M1 and M2.
Figure 3. Study depicting activity profiles of WT and truncation mutants of TfCel9B using PASC as a substrate. For each time course, increasing concentrations of TfCel9B were incubated on 1 mg/ml PASC in 0.6 mL 50 mM sodium acetate used as buffer and µmoles of product were released. Solid lines were derived using polynomial curves that best fit the data. Upper portion, activity profiles of mutants Fn3 and ΔFn3; middle portion, mutants E9B, ΔE9B and cd9B. Bottom portion; WT, mutants M1 and M2.
Figure 4. Study depicting activity profiles of WT and truncation mutants of TfCel9B using xylan as a substrate. For each time course, increasing concentrations of TfCel9B were incubated on 1 mg/ml xylan in 0.6 mL 50 mM sodium acetate used as buffer and µmoles of product were released. Solid lines were derived using polynomial curves that best fit the data. Upper portion, activity profiles of mutants Fn3 and ΔFn3; middle portion, mutants E9B, ΔE9B and cd9B. Bottom portion; WT, mutants M1 and M2.
Figure 5. Activity Assay of WT and truncation mutants on BMCC. Study depicting activity profiles of WT and truncation mutants of TfCel9B using BMCC as a substrate and µmoles of product were released. For each time course, increasing concentrations of TfCel9B were incubated on 1 mg/ml BMCC in 0.6 mL 50 mM sodium acetate used as buffer. Solid lines were derived using polynomial curves that best fit the data. Upper portion, activity profiles of mutants Fn3 and ΔFn3; middle portion, mutants E9B, ΔE9B and cd9B. Bottom portion; WT, mutants M1 and M2.
Figure 6. Study depicting activity profiles of WT and truncation mutants of TfCel9B using xyloglucan as a substrate. For each time course, increasing concentrations of TfCel9B were incubated on 1 mg/ml xyloglucan in 0.6 mL 50 mM sodium acetate used as buffer, and micromoles of glucose were released. Solid lines were derived using polynomial curves that best fit the data. Upper portion, activity profiles of mutants Fn3 and ΔFn3; middle portion, mutants E9B, ΔE9B and cd9B. Bottom portion; WT, mutants M1 and M2.
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