Figure Legend
Figure 1. Upstream process flowsheet for the two-stage
semicontinuous operation base case scenario in the SuperPro Designer®
model.
Figure 2. Two-stage semicontinuous operation of transgenic rice
cell culture with the RAmy3D expression system.
Figure 3. Downstream process flowsheet for the two-stage
semicontinuous operation base case scenario in the SuperPro Designer®
model.
Figure 4. Results of the base case scenario analysis. (A)
Tornado chart displaying sensitivity of rrBChE cost of goods sold (COGS)
to variation in the tested process parameters over the selected analysis
range. Individual scenario analyses of (B) expression level, (C) harvest
size, and (D) Hupresin capacity variation on rrBChE COGS. (E) Variation
in COGS breakdown in the three scenario analyses. The simulated facility
is re-sized for each scenario analysis result to maintain base case
production level and concentration in product formulation. Values
corresponding to the base case are circled in black. FW, fresh weight.
Figure 5. Annual operating expenditures for production of
rrBChE in two-stage semicontinuous and two-stage batch mode operation
using a contract manufacturing organization and with a greenfield
single-product facility broken down by (A) cost items, and (B)
manufacturing section. Depreciation costs are not included in the annual
operating costs for the new facility scenarios.
Figure 6. Total equipment costs for a greenfield single-product
facility producing rrBChE in two-stage semicontinuous and two-stage
batch mode operation broken down by manufacturing section.
Figure 7. (A) Sensitivity analysis of rrBChE cost of goods sold
to the cost of culture media for batch mode operation using two-stage
(medium exchange) and single-stage (simple) induction strategies for new
greenfield single-product facility design including depreciation costs.
(B) The variation in operating cost contributions as a function of
culture medium cost. The base case scenario price is at $0.10/L. PBR,
production bioreactor.