Fig 3. The ApHRC expression level in salivary glands of
pea aphids.
(a) Heat map of differentially expressed genes (DEGs) in the salivary
gland of Serratia -free aphid (Serratia -) andSerratia -infected aphid (Serratia +). (b) The
relative expression of the top 10 up-regulated candidate secretary
saliva proteins in Serratia -infected salivary glands
transcriptome when compared with Serratia -free aphid. Gene in the
red box is ApHRC . Three biological replications were conducted in
the RNA-seq analysis. (c) FISH to detect the ApHRC expression
level in aphid salivary glands. ApHRC mRNA was hybridized with 5-
carboxyfluorescein (5-FAM) in red, nuclei were stained with
4’,6-diamidino-2-phenylindole (DAPI) in blue. PG, principal gland; AG,
accessory gland. Bars = 50 μm. (d) ApHRC relative expression
level in aphid body (n=6). (e) ApHRC relative expression level in
aphid salivary gland (n=6). The data shown are mean ± standard error
(SEM). * above the bars indicate significant differences among different
treatments at P < 0.05 (t-test, *P < 0.05, **P
< 0.01, ***P < 0.001).