Fig 3. The ApHRC expression level in salivary glands of pea aphids.
(a) Heat map of differentially expressed genes (DEGs) in the salivary gland of Serratia -free aphid (Serratia -) andSerratia -infected aphid (Serratia +). (b) The relative expression of the top 10 up-regulated candidate secretary saliva proteins in Serratia -infected salivary glands transcriptome when compared with Serratia -free aphid. Gene in the red box is ApHRC . Three biological replications were conducted in the RNA-seq analysis. (c) FISH to detect the ApHRC expression level in aphid salivary glands. ApHRC mRNA was hybridized with 5- carboxyfluorescein (5-FAM) in red, nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in blue. PG, principal gland; AG, accessory gland. Bars = 50 μm. (d) ApHRC relative expression level in aphid body (n=6). (e) ApHRC relative expression level in aphid salivary gland (n=6). The data shown are mean ± standard error (SEM). * above the bars indicate significant differences among different treatments at P < 0.05 (t-test, *P < 0.05, **P < 0.01, ***P < 0.001).