Table 2 . Specific
formation rates of Hlip, AMP and Lip-AMP in different
assays. voverall represents values from assay of
the overall lipoylation reaction (Fig.1); vStep1gives rates from assay of the first reaction step without addition of
Hapo; vStep2 gives rates from
assay of the second reaction step with the use of Lip-AMP (236 µM)
formed from the first reaction as substrate. The initial concentration
of Hapo was 10 µM for the overall reaction and the Step
2 reaction.