2.1 Chemicals and Reagents
NaCl, Tris, and HCl were of analytical grade and purchased from
Sinopharm Chemical Reagent Co. LTD (Beijing, China). ATP, ADP, AMP and
lipoic acid were purchased from Sigma-Aldrich (Shanghai, China). The
bicinchoninic acid (BCA) Protein Assay Kit was purchased from Beijing
Solarbio Science & Technology Co. LTD (Beijing, China). Acetonitrile
and trifluoroacetic acid (TFA) were of chromatographic grade and
purchased from J&K Scientific Ltd. (Beijing, China). Chemically
competent cells of E. coli TOP10 and E. coli BL21(DE3)
were purchased from Weidishengwu Ltd. (Beijing, China). In-fusion
cloning was used for the ligation of sequence fragments to vector with
the In-fusion HD Cloning Kit (Clontech Laboratories, Inc, US).
Luria-Bertani (LB) liquid medium (tryptone 10 g/L, yeast extract 5 g/L
and NaCl 10 g/L) and solid medium (1.5% agar) with kanamycin (100
μg/mL), ampicillin (100 μg/mL) were used for transformation, screening,
and cell growth.