2.6 Determination of influences of different forms of H protein
Different forms of H proteins (Hapo, Hlip, HK64A) were used to investigate their effects on the reactions catalyzed by LplA. The reaction mixture (200 µL) contained 2 mM lipoic acid, 2 mM ATP, 2 mM MgCl2, 10 μΜ LplA, Tris-HCl (50 mM, pH 7.0) and different concentrations of H proteins (from 0.4 to 400 μM). The components except ATP were premixed and centrifuged before the reactions were initiated by adding ATP to the reaction mixture.