Figure legends:
Figure 1: Schematic of phage display technique carried out for peptide
ligand discovery for AdP.
Figure 2: Fluorescence polarization binding curves for 6 peptides
obtained by phage display against AdP with peptide P10 (inset)
exhibiting the highest binding.
Figure 3: The binding of P10-ELP to AdP at various molar ratios of
P10-ELP to AdP at pH 7.4.
Figure 4: (a) Binding of P10-ELP to AdP at different pH values (3, 4, 5
and 7.4) at a ratio of 2:1 of P10-ELP to AdP. (b) Binding of P10-ELP to
AdP at various molar ratios of P10-ELP to AdP at pH 4.0.
Figure 5: (a) Effect of three different modifiers tested one at a time
on the elution step recovery of AdP in pure component P10-ELP-AdP
binding and precipitation experiments. (b) Effect of arginine
concentration at different pH values on the elution step recovery of
AdP.
Figure 6: SDS PAGE analysis of the purification of AdP from E.
coli lysate.
Figure 7: C4-RP-UPLC chromatograms of AdP spiked into E. colilysate (top) and purified AdP after single step purification using
P10-ELP (bottom).