Association of endotypes to phenotypes in the negative/ low IL-5
levels cluster group:
Nasal polyps were present in clusters 1-4 between 0% in cluster-2 to
31% in cluster-4. Asthma, but not N-ERD, was reported in 0 to 23% in
clusters 1 to 4.
Cluster group with high levels of IL-5 consisted of clusters
5-7 :
The concentration of IL-5 and also total IgE values were significantly
elevated in more than in three other clusters in cluster-5 and more than
in five other clusters in clusters 6 and 7, ECP was significantly
elevated in > 5 other clusters in cluster-5 and 7 and
significantly elevated in > than in three other clusters in
cluster-6.
In cluster-5 (7 CRS cases) , the levels of type-2 related
chemokines eotaxin/ CCL11, TARC/ CCL17, and PARC/ CCL18 were
significantly higher than in > 5 other clusters. Also,
the Th1/ Th2 chemokines MCP-3/ CCL7 and MIP-1α/ CCL3 were
significantly higher than > 5 other clusters.
IP-10/CXCL10 and ENA-78/CXCL5 did not differ between the clusters. MPO
and IL-22 were significantly elevated (> 5 other
clusters), and IFNγ (not included in the cluster analysis) was present
in cluster-5 (strong Th1/Th2 pattern).
- In cluster-6 (16 CRS cases) , the eotaxin/ CCL11
(>than five other clusters), PARC/CCL18, and TARC/CCL17
levels (> 3 other clusters) were significantly elevated.
SE-IgE was also significantly elevated (> 5 other
clusters). MPO was significantly elevated (> 2 other
clusters), and IFN−γ (not included in the cluster analysis) was
present in cluster-6 (type 2-inflammatory pattern ).
- In cluster-7 (2 CRS cases) , the chemokines TARC/CCL17,
PARC/CCL18 were > 5 times and eotaxin/CCL11
> 3 times significantly higher than other clusters. All
other chemokines revealed no significant differences. SE-IgE was also
significantly elevated in >5 other clusters. MPO
(included in the cluster analysis) was significantly elevated, and
TNFα was present in cluster-7 (type 2-inflammatory pattern ).