Fig. 1: (a) Multiple sequence alignment of PEBP-like
protein sequences. Sequences were aligned using the MUSCLE alignment
tool. Sequences are aligned to the 81-172 amino acid long region of FT
protein from Arabidopsis. The marked residues/conserved domains are
required for the corresponding activity of FT and TFL1-like sequences.
The band at the bottom of the alignment represents the percentage of
conservation of the amino acids at a position. Red indicates stronger
conservation while blue represents variable sequences. (b)Phylogenetic reconstruction of the PEBP-gene family with
homologous C. pallens sequences . The evolutionary history was
inferred by using the Maximum Likelihood method based on the JTT
matrix-based model. The bootstrap consensus tree inferred from 1000
replicates is taken to represent the evolutionary history of the taxa
analyzed. Aly: Arabidopsis lyrata subsp. lyrata, Ath:Arabidopsis thaliana , Ac: Allium cepa, Bv: Beta
vulgaris, Bd: Brachypodium distachyon, Cs:Chrysanthemum seticuspe , Chm: Chrysanthemum X morifolium,Gm: Glycine max, Ha: Helianthus annuus, Nt:Nicotiana tabacum , Osa: Oryza sativa, Ptr:Populus trichocarpa, ZCN: Zea mays and C. pallens .
Branches in red represent the MFT clade, blue represents theTFL1 clade
and green represents the FT clade. (c) Functional
characterization of the C. pallens PEBP- like sequences. The
number of rosette leaves of the transgenic and the wild-type plants at
flowering (mean ± SD, n = 10). (Significance of transgenic and control
plants compared to Ler ft-1 , Student’s t-test *** P <
0.001, ** P <0.01). The x-axis represents Ler as the control
plant, Ler ft-1, as the mutant, and the transgenic lines
transformed with FT/TFL1 -like sequences from C. pallenscloned into the binary vector pB2GW7 with the AtSUC2 promoter.