Angiogenic factors
Maternal serum concentrations of PLGF and sFLT-1 in pg/mL were measured from thawed samples using an automated biochemical analyser (BRAHMS KRYPTOR compact PLUS; Thermo Fisher Scientific, Hennigsdorf, Germany). In this analyser, the inter-assay coefficients of variation for the low and high concentrations were 22% and 5% for PlGF and 5% and 5% for sFLT-1, respectively. Assays cover a measurement range from 3.6-7000 pg/mL for PlGF and from 22-90,000pg/mL for sFLT-1.