VCAM
Serum concentrations of VCAM were determined using the Quantikine VCAM ELISA kit (Biotechne, R&D Systems, Oxon, UK). The detection limit was 0.60 μg/L with a measurement range 349 to 991 μg/L. The intra-assay coefficients of variation for the low and high concentrations were 2.3% and 3.6%. The inter-assay coefficients of variation for the low and high concentrations were 7.8% and 5.5%.