Transcriptome sequencing
SARS-coV-2 affects the cardiovascular system to a great extent6 and to understand the effects of thalidomide and its
derivatives, human umbilical vein endothelial cells (HUVEC) were
subjected to 20µM thalidomide or 20µM lenalidomide or 20µM pomalidomide
or vehicle control treatment for 8h. RNA was isolated using TRIzol®
method and whole transcriptome sequencing was performed using Illumina
HiSeq 2500 platform. The data can be accessed at GEO with the Accession
ID GSE118979. The sequence reads were aligned with reference genome ofHomo sapiens using TopHat2 (v2.0.8) and the followed by
transcript compilation and gene identification was done using Cufflinks
(v2.2.0) 24. The DEG were identified using Cuffdiff
program (v2.2.0) 24.