3.5.2 Intra-signalling pathway of Nox2 activation by LPS
To investigate upstream pathways implicated in Nox2 activation we
analysed the role of AKT, which is implicated in
p47phox activation and ultimately Nox2
up-regulation.21 Betamethasone and
methylprednisolone-treated cells stimulated with LPS showed a
significant decrease of AKT phosphorylation compared to LPS-treated
cells alone (Figures 6, A and B). This effect was dose-dependent with a
significance reached at concentrations of 300 and 600 ng/ml compared to
150 ng/ml (Figure 6, A and B). Similarly, betamethasone and
methylprednisolone-treated cells stimulated with LPS reduced
p47phox phosphorylation compared to LPS-treated cells
alone, an effect reached with 300 and 600 ng/ml of betamethasone and
methylprednisolone (Figures 6, C and D).