B cell and DC activation analysis early after immunization
3, 6 and 9 days (D3, D6, D9) after the first immunization, the inguinal lymph nodes of 3 mice in each group were collected and disrupted with the plunger of a syringe. The cell suspensions were stained with anti-CD11c (PE-Cy7), anti-MHCII (PE), anti-CD19 (APC) and anti-CD40 (FITC) antibodies. Data were acquired on a FACSCalibur flow cytometer (BD Biosciences, Franklin, CA, USA).