Sensitization of mice.
For oral sensitization, mice received 1 mg of cashew protein extract
supplemented with 10 µg of Cholera Toxin (List Biological Laboratories)
per intragastric route, at a rate of one intragastric administration per
week for 6 consecutive weeks. For skin sensitization, mice received 100
μg of cashew protein extract on the skin of the lower back. A liquid
solution was deposited on a gauze that was maintained on depilated skin
with Tegaderm® (3M) for 72 hours. Before each
application, skin was pre-treated using a dermatological laser (Pantec
Biosolutions) at a fluence of 5.5 Joules/cm² to mimic atopic skin. Mice
received allergen twice a week, every two weeks, for 7 weeks.