Injection of anti IgE and IgG receptor blocking antibodies.
Mice received 100 µg of anti-IgE (clone EM-95, kindly provided by Fred
Finkelman), 100 µg of anti-FcγRII/RIII (clone 2.4G2, Bio X Cell) or a
mix of rat IgG2b and rat IgG2a as isotype controls (clones LTF-2 and
2A3, respectively, Bio X Cell) by intraperitoneal route. To avoid any
non-specific anaphylactic reaction, all mice received 200 µg of
triprolidine hydrochloride (Sigma) by intraperitoneal route 30 minutes
before injection of the monoclonal antibodies. Mice were challenged
orally 24 hours later.