Sensitization of mice.
For oral sensitization, mice received 1 mg of cashew protein extract supplemented with 10 µg of Cholera Toxin (List Biological Laboratories) per intragastric route, at a rate of one intragastric administration per week for 6 consecutive weeks. For skin sensitization, mice received 100 μg of cashew protein extract on the skin of the lower back. A liquid solution was deposited on a gauze that was maintained on depilated skin with Tegaderm® (3M) for 72 hours. Before each application, skin was pre-treated using a dermatological laser (Pantec Biosolutions) at a fluence of 5.5 Joules/cm² to mimic atopic skin. Mice received allergen twice a week, every two weeks, for 7 weeks.