Figure legends
Figure 1. Increased single-cell transcriptome heterogeneity of
suspension-cultured CHO cells over time. Single-cell transcriptome
sequencing was performed on 85, 70, and 78 cells from cultures
maintained for 4, 8, and 11 days, respectively. Principal components 1
(proportion of variance, 0.31) and 2 (proportion of variance, 0.077) are
plotted. Blue, black, and red plots represent individual day 4, day 8,
and day 11 cells, respectively. Variances in principal component 1
between the different culture times had a p-value < 2.2e-16.
Figure 2. Single-cell resolution mitochondrial genomic
heterogeneity in suspension-cultured CHO cells. Genotypes of 498
variable loci in each cell were visualized.
Figure 3. Correlation between mutation rate and culture time.
Single-cell resolution genomic mutation analysis was performed on the
mitochondrial genomes of CHO cells cultured for different periods. No
statistical correlation was observed between culture time (Days) and the
heteroplasmic variant rate (rate of new mutations at called variable
loci; p = 0.14).
Figure 4. Cell cycle–dependent subpopulations in traditional
adherent CHO cultures. Single-cell transcriptome sequencing was
performed on 24 G1 cells (circles) and 8 G2 cells (crosses).
Figure 5. Hidden subpopulations in suspension cultures of CHO
K1 cells. Individual D4 cells clustered into groups with high enolase
expression (2000–4200 RPM, red) and low enolase expression (0–2000
RPM, black). (a) Histogram of enolase expression degree (RPM) in
suspension CHO K1 cells after 4 days. (b) A colored plot of principal
components 1 and 2 in Day 4 suspension cultures of CHO K1 cells.