Supplemental Figure and Table legends
FIGURE S1 Timelines of set air temperatures and measured leaf temperatures for experiments under the LLW (low light & warm temperature), LLC (low light & cool temperature), HLW (high light & warm temperature), and HLC (high light & cool temperature) growth conditions.
FIGURE S2 Log2 gene expression (via RNAseq) for genes from the heat map in Figure 4a that are a part of the photosynthesis GO category (GO:0015979). Genes that are more strongly induced in IT in response to HLC growth conditions relative to the response of SW to HLC conditions, such as the chloroplastic glucose-6P/phosphate transporter GPT2 (AT1G61800), the cytosolic fumarase FUM2 (AT5G76550), the chloroplastic, the phosphofructokinase AT1G76550, the ferritin FER4 ( AT2G30400), the chloroplastic Na+ efflux regulator KEA2(AT4G00630), and the high-light inducible LHC2-component LHCB4.3(AT2G40100), are located at the top of table. Whereas genes that are more strongly repressed in HLC conditions in the IT ecotype relative to the SW ecotype in HLC conditions can be found at the bottom of the table, such as LHC2-components LHCB1.4 (AT2G34430), LHCB3(AT5G54270), LHCB1.5 (AT2G34420), and LHCB1.1(AT1G29920), PSII component PSBP-2 (AT2G30790), and triose phosphate transporter TPT (AT5G46110).
Table S1. The 1415 genes up-regulated in HLC-grown SW plants (minimum fold change of 2 and adjusted P -value of < 0.01).
Table S2. The 1992 genes up-regulated in HLC-grown IT plants (minimum fold change of 2 and adjusted P -value of < 0.01).
Table S3. The 1531 genes down-regulated in the HLC-grown SW plants (minimum fold change of 2 and adjusted P -value of < 0.01).
Table S4. The 1671 genes down-regulated in HLC-grown IT plants (minimum fold change of 2 and adjusted P -value of < 0.01).
Table S5 . The 1090 genes up-regulated in both HLC-grown IT and SW plants (minimum fold change of 2 and adjusted P -value of < 0.01)
Table S6. The 804 genes down-regulated in both HLC-grown IT and SW plants (minimum fold change of 2 and adjusted P -value of < 0.01).
Table S7. The 356 genes that co-clustered into the cluster labeled “HLC-Specific Group 1” and “HLC-Specific Group 2” in Fig. 4a.
Table S8. PANTHER Overrepresentation Test (Released 2019-07-11) on GO Ontology database (Released 2019-12-09) for genes up-regulated in both HLC-grown IT and SW plants.
Table S9. PANTHER Overrepresentation Test (Released 2019-07-11) on GO Ontology database (Released 2019-12-09) for genes down-regulated in both HLC-grown IT and SW plants.
Table S10. SW-HLC differentially regulated genes with induction in SW ecotype was at least two-folder greater than induction in the IT ecotype.
Table S11. PANTHER Overrepresentation Test (Released 2019-07-11) on GO Ontology database (Released 2019-12-09) for genes induced more strongly in SW ecotype.
Table S12. SW-HLC differentially regulated genes with down-regulation in SW ecotype was at least two-folder greater than down-regulation in the IT ecotype.
Table S13. IT-HLC differentially regulated genes with induction in IT ecotype was at least two-folder greater than induction in the SW ecotype.
Table S14. PANTHER Overrepresentation Test (Released 2019-07-11) on GO Ontology database (Released 2019-12-09) for genes induced more strongly in IT ecotype.
Table S15. IT-HLC differentially regulated genes with downregulation in IT ecotype was at least two-folder greater than downregulation in the SW ecotype.
Table S16. PANTHER Overrepresentation Test (Released 2019-07-11) on GO Ontology database (Released 2019-12-09) for genes induced more downregulated in IT ecotype.
Table S17. Genes previously reported to be CBF1–3-regulated—Comparison of transcriptomic data from four growth conditions for both ecotypes to genes identified as having diminished induction in it:cbf123 and sw:cbf123 mutants following a 24-hour 4°C treatment by Park, Gilmour, Grumet, & Thomashow (2018).
Table S18. Comparison of transcriptomic data from four growth conditions for both ecotypes to genes reported to be over-expressed under low light and warm temperatures by Park, Gilmour, Grumet, & Thomashow (2018).
Table S19. Data collected on sw:cbf2 mutant (RT-qPCR, leaf thickness, photosynthetic capacity, electrolyte leakage, freezing survival, chlorophyll per unit area, rosette diameter).