Phylogenetic analysis of B646L, E183L and EP402Rgenes of African swine fever virus detected and sequence in samples collected
The targeted fragments of the ASFV samples was amplified and sequenced successfully as follows B646L (13), E183L (10) andB602L (10) genes. While only 6 ASFV samples were successfully amplified and sequenced for the EP402R . The samples were selected from the LPMs as follows Katsit (8), Dawaki (3), Pandam (1) and Numan (1).
The phylogeny of B646L gene revealed ASFV genotype I was circulating in all the selected LPMs within the study period (Fig 2).Phylogenetic analysis of the E183L ( p54) gene of ASFV revealed that live pig market ASFV sequences clustered with genotype 1a sequences of Nigeria and West African countries (Fig 3). Furthermore, the phylogeny of theEP402R (CD2v) gene shows all the ASFV sequences from Nigeria clustered within serogroup 4 alongside isolates from Mali (Fig 5)