2.5 Transfection
After 24-h incubation in serum-free medium, VSMCs were transfected with the miR-26a mimic (JTS Scientific, China) or miR-26a inhibitor (JTS Scientific, China) or a corresponding negative control (NC) or siRNA (Wanleibio, China) or SB431542 (MCE, China) according to the manufacturer’s instructions. After 24- or 48-h transfection, cells were treated with AngII (10-7 mol/L for 24 h).