2.3 Immunohistochemical staining
Aortic tissue sections (4 µm) were deparaffinized, and endogenous
peroxidase activity was quenched with
H2O2. The sections were incubated with
anti-α-actin (dilution 1:200) and anti-proliferating cell nuclear
antigen (PCNA; dilution 1:100) antibody at 4°C overnight, then with
secondary antibody. After staining, each section was analyzed by
confocal microscopy (DS-Fi1-Eclipse, Nikon).