2.14 Data and statistical analysis
The data and statistical analysis comply with the recommendations of the British Journal of Pharmacology on experimental design and analysis in pharmacology (Curtis et al. , 2018). All data are expressed as mean ± SD and GraphPad Prism 6 software was performed to statistical analysis. To determine differences between groups with one factor, data were tested using unpaired Student’s t-test for two group’s comparison, and one‐way ANOVA with Tukey’s post hoc test analysis for multiple group comparisons. To determine differences between groups with multiple factors, data were tested using two-way ANOVA followed by Bonferroni’s test. Post hoc tests were run only when F achieved P < 0.05. Differences were considered statistically significant at P < 0.05. All measurements were undertaken only for n ≥ 5.