2.7 Maintenance & Monitorization
The neurons on a chip are incubated in darkness for 22 days in vitro (DIV) under controlled temperature (29°C) and humidity (70%). After seven days it is needed to refill the microfluidic system with another syringe. The used workflow is estimated from a previous work in the study of neural network formation on top of typical plate cultures.
The data reported in this study correspond to 2 cultures grown under the same conditions. Culture visualization and Network process monitorization are performed using a charge-coupled device camera (DSFi1, Nikon) mounted on a phase-contrast microscope (Eclipse TiS, Nikon) with a 10x air objective (Achromat, ADL, NA 0.25) and an automated motorised XYZ stage controller. Mosaic images are captured in a daily basis with the large image method implemented in NIS-Elements software, which does automatic blended stitching with an overlap of 25%.