2.3.3 White blood cells morphology
A peripheral blood film (made from a drop of blood from an EDTA anticoagulated tube or skin puncture) is stained with Wright stain and microscopically examined using scanning (10x), and oil (100x) objectives. The WBC morphology was carefully evaluated using a 100x microscope objective.
2.4 Statistic analyses. Results were expressed as mean values ± SEM. All data were statistically analyzed using one-way analysis of variance (ANOVA) for repeated measures, followed by post hoc Bonferroni’s test. All analytical procedures were performed using the scientific statistic software GraphPad Prism 5 (GraphPad Scientific, San Diego, CA, USA). Differences of P˂0.05 were considered as statistically significant.