2.3.3 White blood cells morphology
A peripheral blood film (made from a drop of blood from an EDTA
anticoagulated tube or skin puncture) is stained with Wright stain and
microscopically examined using scanning (10x), and oil (100x)
objectives. The WBC morphology was carefully evaluated using a 100x
microscope objective.
2.4 Statistic analyses. Results were expressed as mean values ±
SEM. All data were statistically analyzed using one-way analysis of
variance (ANOVA) for repeated measures, followed by post hoc
Bonferroni’s test. All analytical procedures were performed using the
scientific statistic software GraphPad Prism 5 (GraphPad Scientific, San
Diego, CA, USA). Differences of P˂0.05 were considered as statistically
significant.