Figure legends
Fig. 1 Eggs (A), larva (B), cocoons (C) and adult (D) of the
peach fruit moth Carposina sasakii (A-D) and the damage
symptoms to apple (E-G). The hatched larva bores into apple usually near
the calyx with white secreta near the boring hole (E); the damaged apple
showing shrinkage (F); damage from larvae boring and developing in the
apple (G).
Fig. 2 Genome features of Carposina sasakii. (a)
GenomeScope analysis of genome size, heterozygosity and duplicate rate
using k-mers (K = 17) count histogram, indicating a genome size of
338.52 Mb, a heterozygosity of 1.06%, and a duplication rate of 2.06%;
(b) Genome-wide all-by-all Hi-C interaction identified 32 linkage
groups; (c) Synteny between Carposina sasakii (Csas) andCydia pomonella (Cpom) and Spodoptera litura (Slit)
genomes reveal highly conserved gene order and chromosomal fusion or
split events in the three moths.
Fig. 3 Comparative genomics of Carposina sasakii. (a)
Phylogenetic tree of PFM with 15 insect genomes including 11 other
Lepidoptera. The phylogeny was inferred from 947 single-copy genes with
364,262 reliable sites by an approximately-maximum-likelihood method.
All nodes received bootstrap support of 100. (b) Orthogroups shared by
five Lepidoptera species of Carposina sasakii ,Cydia pomonella , Bombyx mori , Ostrinia
furnacalis and Helicoverpa armigera.
Fig. 4 Schematic arrangement of the domains of five circadian
genes including period (PER), timeless (TIM), Clock(CLK), cycle (CYC) and cryptochrome (CRY-m, CRY-d) inCarposina sasakii and other 15 insects. Boxes in different color
show different domains, while numbers under boxes show the postion of
domains on protein sequences. Species and their taxonomic status are
shown on the left: Tcas, Tribolium castaneum ; Agla,Anoplophora glabripennis ; Agam, Anopheles gambiae ; Dmel,Drosophila melanogaster ; Pxy, Plutella xylostella ; Cpom,Cydia pomonella ; Csas, Carposina sasakii ; Dple,Danaus plexippus ; Tni, Trichoplusia ni ; Slit,Spodoptera litura ; Harm, Helicoverpa armigera ; Bmor,Bombyx mori ; Bman, Bombyx mandarina ; Msex, Manduca
sexta ; Gmel, Galleria mellonella ; Ofur, Ostrinia
furnacalis .
Fig. 5 Phylogenetic relationships of two PAS domains in three
circadian genes: period (per), Clock (clk) andcycle (cyc). Each tip is labeled by the name of domain, gene and
species. Abbreviations of species are same as in Fig. 4. Six clades
shaded in different color reveal two domains of three genes, while one
domain of clk gene has two different type among species. Tips in
red show the position of Carposina sasakii .