2.2 Necropsy and Histopathology
After death, three (22388PT20, 22390PT20 and 22391PT20) of the five rabbits were necropsied according to routine procedures. Tissue samples were collected for histopathology (eyelid, genitalia, ear, lung and kidney) and virology (liver, spleen, lung, eyelid and genitalia). The other two rabbits were autolysed, therefore only submitted to virological examination.
For histopathology, liver, spleen, kidney, eyelid, ear, muzzle and lung fragments were fixated in 10% (w/v) neutral buffered formalin, routinely paraffin embedded, sectioned at 4 µm, and stained with Haematoxylin and Eosin (H&E).