Materials and Sequencing
The experimental animals in this project were collected from the
spawning field of G. przewalskii (Figure 1C) in Quanji river, a
tributary of Qinghai Lake, with an altitude of 3,194 meters (Figure 1A
and B). Muscle was used for DNA extraction and then high quality DNA was
constructed libraries and sequenced on Pacbio Sequel and Illumnia X-ten
Platform for genome de novo assembly. DNA from blood was
extracted and used for constructing Hi-C libraries. For genome
annotation, we sequenced RNA by Illumnia X-ten and Pacbio sequel for
RNA-seq and Iso-seq data. All the RNA was extracted from brain and gill.