Materials and Sequencing
The experimental animals in this project were collected from the spawning field of G. przewalskii (Figure 1C) in Quanji river, a tributary of Qinghai Lake, with an altitude of 3,194 meters (Figure 1A and B). Muscle was used for DNA extraction and then high quality DNA was constructed libraries and sequenced on Pacbio Sequel and Illumnia X-ten Platform for genome de novo assembly. DNA from blood was extracted and used for constructing Hi-C libraries. For genome annotation, we sequenced RNA by Illumnia X-ten and Pacbio sequel for RNA-seq and Iso-seq data. All the RNA was extracted from brain and gill.