Methods
To address the challenges identified above, our combined process consists of three areas: 1) discovery of single nucleotide polymorphisms in segregating in white sturgeon using reduced representation genomic library sequencing, including filtering steps to improve the likelihood that candidate markers represent single-locus variants and the lowest functional ploidy (somy) of the target species, 2) verification that the markers conform to the expected polysomic segregation and inheritance by examining allele count ratios in a large set of samples and the incidence of Mendelian incompatibilities in a set of known parent-offspring trios, and 3) evaluating the utility of these markers to resolve population genetic parameters via the accuracy of inferred relationships and relatedness and the precision of population statistic estimates.