Methods
To address the challenges identified above, our combined process
consists of three areas: 1) discovery of single nucleotide polymorphisms
in segregating in white sturgeon using reduced representation genomic
library sequencing, including filtering steps to improve the likelihood
that candidate markers represent single-locus variants and the lowest
functional ploidy (somy) of the target species, 2) verification that the
markers conform to the expected polysomic segregation and inheritance by
examining allele count ratios in a large set of samples and the
incidence of Mendelian incompatibilities in a set of known
parent-offspring trios, and 3) evaluating the utility of these markers
to resolve population genetic parameters via the accuracy of inferred
relationships and relatedness and the precision of population statistic
estimates.