2.2. Plasma IgG
Venoglobulin IH5 (50 mg/mL) provided by Japan Blood Products Organization (JBPO) was diluted with 20 mM sodium acetate, 100 mM NaCl, pH 6 buffer, which is a relevant column chromatography flow through buffer condition for plasma IgG, to produce 15 mg/mL plasma IgG solution for use in aggregate spiking experiments and 11 mg/mL for use as a reference solution for filtration.