Blood lactate and s100β measurements:
Lactate levels were studied before, during and after ASCP (during the
first minute) from the left radial artery cannula. Samples were also
taken in the 2nd and 6thpostoperative hour. During ASCP an additional blood sample was taken
from descending aorta to correlate with the radial artery sample. For
s100β determination blood samples were obtained from descending aorta
just before terminating ASCP and from radial artery in the
6th postoperative hour. Kits obtained from Cloud Clone
Human ELİSA were used for s100β determination. All blood samples were
centrifuged for 20 minutes with 1000 cycles/minute and the sera obtained
were kept at 80°C to be dissolved all at once.