2.6.5. SEC-HPLC
Cell culture supernatant and a SXC-purified sample, previously filtered
using 0.45-µm syringe filters, were analyzed in a LC-20 Prominence HPLC
system (Shimadzu, Japan) fitted with a TSKgel G3000SWXL column (7.8 mm
ID x 30 cm, stainless steel). 30-µL samples were applied to the column
and the analyses were carried out at 1 mL/min using a 12.5 mM phosphate
buffer with 250 mM NaCl at pH 7.0.