2.6.2. ELISA for VLP quantification
A sandwich ELISA previously described (Lima et al., 2019) was used to quantify the VLPs in the chromatography fractions. Briefly, pan-flavivirus 4G2 antibody was used to coat plates, whereas in-house biotinylated 4G2 was used as a secondary antibody followed by avidin-HRP. TMB Single Solution (Invitrogen, USA) was used for detection, reaction was stopped with 1 N HCl, and measured at 450 nm.