Genome size estimation and genome assembly
The Kmer-based method of the Illumina short read data was used to analyze the genome survey to estimate the genome size, heterozygosity, and repeat content of the burbot genome (Liu et al., 2013). The NextDenovo package (https://github.com/Nextomics/NextDenovo) was performed to assemble the burbot genome with PacBio long reads by using the following parameters: parallel_jobs, 300; seed_cutoff, 15,000; pa_correction, 320; and random_round, 100. To correct the random sequencing errors in the NextDenovo output, two steps of genome sequence polishing were applied. The arrow was used to polish the genome by using the long sequencing data (Chin et al., 2013), and two rounds of polishing using Illumina short reads were then applied with Pilon (Walker et al., 2014).