Sample and DNA extraction
A single female fish (~800 g) was collected in November
2019 from Heilong River, the northeastern part of China. Muscle, eye,
gonad, gill, liver, and spleen tissues were collected and stored in
liquid nitrogen until DNA and RNA extraction. Muscle tissues were used
for DNA sequencing for genome assembly, while all tissues were used for
transcriptome sequencing. Genomic DNA from the white muscle tissue was
extracted using the standard phenol/chloroform extraction method to
construct the DNA sequencing library. The integrity and concentration of
the genomic DNA molecules were checked using 1% agarose gel
electrophoresis and Pultton DNA/Protein Analyzer (Plextech, USA).