Viruses:
Highly pathogenic PRV JS-2012 variant strain (Tong et al., 2015) was
obtained from Shanghai Veterinary Research Institute, Shanghai, China.
The classical PRV strain Bristol (classical strain), Shope, Becker, and
Bartha strains were available at the National Centre for Foreign Animal
Diseases (NCFAD)-Winnipeg. All these viruses were propagated and
titrated in PK-15 cells. The PRV modified live vaccine AUSKIPRA - GN
(PRV Bartha-K61 strain) was purchased from Laboratorios HIPRA S.A. in
Spain and PR-VAC PLUS (PRV Bucharest strain) was kindly provided by
Zoetis Inc. in Lincoln, NE, USA. PRV genomic DNA was extracted from
tissue culture amplified virus, PRV positive clinical samples, or from
vaccines reconstituted in PBS. Cross reactivity (analytical specificity
or exclusivity) of the PCR with other swine viruses was tested with a
genomic panel comprised of swine viruses extracted from cell culture
amplified African and classical swine fever viruses and foot and mouth
disease virus (FMDV); clinical samples positive for porcine respiratory
and reproductive syndrome virus (PRRSV), porcine epidemic diarrhea virus
(PEDV), and porcine circovirus 2 (PCV2); and herpesviruses originating
in bovine, elk , caprine, Rangifer , and an unknown cervid . PRV
pUC57 plasmid (545 ng/µL) was purchased from Genscript.