DNA isolation and genotyping
For total genomic DNA extraction, leaf tissue was collected from plants
grown as previously described prior to glyphosate treatment. Samples
were immediately frozen in liquid nitrogen and stored at -80°C. Tissue
was ground using a TissueLyser II (Qiagen; 30 Hz for 2 min). Genomic DNA
was extracted from 100 mg fresh weight tissue following a modified
cetyltrimethylammonium bromide (CTAB) extraction protocol (Doyle, 1991).
DNA quality and concentration were measured using a NanoDrop
spectrophotometer (Thermo Scientific ND-1000). All DNA samples were
normalized to 5 ng uL-1 with deionized water.