FCM
To detect the proportion of
CD15+LOX-1+ PMN-MDSCs in PB of NSCLC
patients and HC, ACK buffer was used to lyse RBCs, and the remaining
cells were stained with PE/Cy5.5-anti-human-CD11b (eBioscience,
0.25μg/1×106 cells), APC-anti-human-CD33 (eBioscience,
0.25μg/1×106 cells), PE-anti-human-LOX-1 (eBioscience,
0.25μg/1×106 cells) and FITC-anti-human-CD15 mAbs
(eBioscience, 0.25μg/1×106 cells) at 4ºC for 30 min.
FCM was performed using a FACSCalibur flow cytometer (Becton Dickinson).
Data were analyzed by FlowJo software.