Reverse transcript PCR and quantitative real-time PCR
The RNA isolation, reverse transcript PCR and quantitative real-time PCR (qRT-PCR) were performed as described previously. The sequences for the primers used are: β-Actin: Forward: 5-GAGTGTGGAGACCATCAAGGA-3, Reverse: 5-TGTATTGCTTTGCGTTGGAC-3; ARG1: Forward: 5-CCTTTGCTGACATCCCTAAT-3, Reverse: 5-GATTCTTCCGTTCTTCTTGACT-3.