Origin of samples for DIA assay library construction and RNA sequencing
The O. niloticus stock used in this study originated from Lake Manzala, Egypt, and brought to Israel in the 1970’s. Twelve male fish (159±33 g) were randomly distributed between two 600L tanks. After two weeks acclimation, one group was exposed to a gradual salinity increase of 5 g/kg per day up to a final salinity of 25 g/kg using synthetic sea salt (Red Sea Salt, Eilat, Israel). Fish were fed twice per day, 2% of their body weight (feeding was ceased 24 hours before sample collection). After 24 hours at the final salinity, fish were sacrificed and immediately dissected. Kidney samples were removed and divided into two aliquots, which were immediately snap-frozen in liquid nitrogen and stored at -80°C. One aliquot from each individual was subsequently processed for proteomics, the other was processed for RNAseq.