Genesis clustering
Protein regulation patterns were clustered in a non-biased manner using Genesis 1.8.1 (Sturn, Quackenbush, & Trajanoski, 2002). Proteins with similar regulation patterns in individual replicates were clustered to identify non-significant proteins which nonetheless followed the same regulation patterns as significant proteins. Six clusters were determined to be the ideal number which grouped the majority of all significantly regulated proteins in two groups, one containing most up-regulated proteins and the other containing most down-regulated, as well as isolating the majority of proteins in clusters with regulation patterns that did not follow treatment groupings.