3.6. Scaffold biomaterial composition plays a key role in
cellular functions
To explore the effects of scaffold biomaterials on cellular functions,
the metabolic activity of cultured DPSCs on various scaffolds was
measured using the AB assay. In SA scaffolds, AB reduction (%) was
maintained at the 2D cell culture (Fig. 5). In contrast, the AB
reduction was significantly increased for GOSA1, RGOSA0.5 and RGOSA1
scaffolds compared to SA only scaffolds across all coating conditions. A
significantly higher degree of reduction of the AB dye was observed in
DPSCs grown on PLL-coated graphene-based scaffolds compared to those
cultured on a 2D surface. This highlights the importance of PLL coating
(as previously proved in coating conditions), where the highest AB
reduction percentage was recorded for PLL-coated RGOSA1 as 93.66%±5.88.