3.6. Scaffold biomaterial composition plays a key role in cellular functions
To explore the effects of scaffold biomaterials on cellular functions, the metabolic activity of cultured DPSCs on various scaffolds was measured using the AB assay. In SA scaffolds, AB reduction (%) was maintained at the 2D cell culture (Fig. 5). In contrast, the AB reduction was significantly increased for GOSA1, RGOSA0.5 and RGOSA1 scaffolds compared to SA only scaffolds across all coating conditions. A significantly higher degree of reduction of the AB dye was observed in DPSCs grown on PLL-coated graphene-based scaffolds compared to those cultured on a 2D surface. This highlights the importance of PLL coating (as previously proved in coating conditions), where the highest AB reduction percentage was recorded for PLL-coated RGOSA1 as 93.66%±5.88.