2.5. Sterilization and coating of scaffolds
Before cell seeding, the scaffolds were sterilized with 80% ethanol for 24 hours, then allowed to dry overnight and washed twice with DPBS. For coating, scaffolds were incubated at room temperature in poly-l-lysine (PLL) (10 µg/mL) overnight followed by incubation at 37 °C in laminin (LAM) solution (10 µg/mL) overnight. Non-coated (NC) scaffolds, which were wetted but not incubated with any coating material, served as control.