Figure legends
FIGURE 1 (a) Histological findings of the medulla oblongata of
a piglet with CT. Hematoxylin and eosin (HE) stain. The scale bar
represents 200 µm. (b) Histological findings of the white matter around
the ventriculus lateralis of the cerebrum of a piglet with CT. HE stain.
The scale bar represents 100 µm.
FIGURE 2 (a) Schematic diagram showing the genome organization
of Anna/2020. (b) Similarity plots
of the complete genomes of genotype 1 APPVs (green lines), genotype 2
APPVs (blue lines), and genotype 3 APPVs (pink lines) with Anna/2020 as
the query sequence using a sliding window of 200 nt and a moving step
size of 20 nt. (c) The phylogenetic tree was constructed using the
maximum likelihood method (GTR+G+I model) in MEGA7 with 1000 bootstrap
replicates. The scale bar indicates the corrected genetic distance.
Anna/2020 is indicated in red text. The percent bootstrap support is
indicated by the value at each node; values <70% are omitted.
FIGURE 3 Distribution and occurrence of atypical porcine
pestivirus infection or detection of atypical porcine pestivirus in
Japan.
FIGURE 4 (a) Schematic diagram showing the genome organization
of Anna/2020 and regions of the obtained genome sequences of Miya/2007,
Kago/2018, and Mae/2018. The genome sequence positions of each strain
corresponding to those of Anna/2020. (b) Similarity plots of the
complete genomes of genotype 1 APPVs (green lines), genotype 2 APPVs
(blue lines), genotype 3 APPVs (pink lines), and the partial genomes of
Miya/2007 (brown lines), Kago/2018 (purple lines), and Mae/2018 (dark
green lines) with Anna/2020 as the query sequence using a sliding window
of 200 nt and a moving step size of 20 nt. (c) The phylogenetic trees
were constructed using the partial genome sequences of five regions by
the maximum likelihood method in MEGA7 with 1000 bootstrap replicates.
The scale bar indicates the corrected genetic distance. Anna/2020,
Miya/2007, Kago/2018, and Mae/2018 are indicated in red, brown, purple,
and dark green texts, respectively. The percent bootstrap support is
indicated by the value at each node; values <70% are omitted.