MRD detection
MRD detection was performed by real-time quantitative PCR, and some
common fusion transcripts, such as ETV6/RUNX1, E2A/PBX1, and BCR-ABL1,
were assessed using reverse transcription PCR. Karyotyping screening was
performed using a conventional karyotyping (G-banding) analysis or
fluorescence in situ hybridization (FISH). Monitoring of BM samples for
MRD, common fusion transcripts and IKZF1 Δ2-8/ALB deletions monitoring
of BM sample was carried out every 2–3 months during the first 3 years
after diagnosis. After which these criteria were assessed every 6 months
for 2 years.