Figure Legends
Figure 1. Schematic diagram of the LAMP amplification assay coupled with CRISPR system. The pictures were captured under blue and UV lights by a smartphone camera or gel imaging system.
Figure 2. The efficiency of CRISPR/Cas12a trans-cleavage system induced by ASFV specific crRNAs. The fluorescent signal was collected induced by different crRNAs with ABI QuantStudio 5 (A) and visualized by gel imaging system using UV light (B).
Figure 3. Comparison of the detection limit between CRISPR/Cas12a system and LAMP-CRISPR assay. The fluorescent signals from a series of 10-fold dilutions of dsDNA template p3xFLAG-CMV-7.1-p72 plasmid. 8: 7×108 copies/μl, 7: 7×107 copies/μl, 6: 7×106 copies/μl, 5: 7×105 copies/μl, 4: 7×104copies/μl, 3: 7×103 copies/μl,2: 7×102 copies/μl,1: 7×101copies/μl,0: 7×100 copies/μl. The fluorescent signals of different dilutions were calculated by ABI QuantStudio 5 (A and B) and visualized by gel imaging system using UV light (C and D) after performed with CRISPR/Cas12a system reaction and LAMP-CRISPR assay.
Figure 4. Specificity of LAMP-CRISPR assay for the detection of ASFV. The fluorescent signals were calculated by ABI QuantStudio 5 (A) and visualized by gel imaging system using UV light (B) with DNA viruses ASFV and PCV2, cDNA of CSFV, FMDV, SVA, PEDV and PRRSV by performing LAMP-CRISPR assay.
Figure 5. Detection of ASFV in swine samples. Samples from nasal swab (A and a), spleen (B and b), liver (C and c), lung (D and d), submandibular lymph node (E and e) and kidney (F and f) were tested by LAMP-CRISPR assay, respectively, and the fluorescent signals were calculated by ABI QuantStudio 5 and visualized by gel imaging system using UV light. Besides, S5 from nasal swabs was not available, which was shown in empty tube from the picture a.