3.4 Specificity test of LAMP coupled with CRISPR Cas12a
To examine the specificity of LAMP-CRISPR detection of ASFV against other swine pathogens, including CSFV, FMDV, SVA, PCV2, PEDV and PRRSV, the tests were performed for detection of the above-mentioned antigens. The results showed that all of these tested swine pathogens were negative, while only ASFV was positive (Figure 4), which demonstrated this method is highly specific for the detection of ASFV.